Formalin-fixed and paraffin-embedded tissue samples (FFPE) have been an important part of research and therapeutic applications for decades.
FFPE is a form of storage and preparation of biopsy specimens that aids in research, experimental research, and diagnosis/drug development. There are many companies like Geneticist Inc which provide complete information about ffpe block storage.
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First, the tissue sample is preserved by binding it in formaldehyde, also known as formalin, to preserve proteins and vital structures in the tissue. It is then embedded in a block of paraffin wax.
This makes it easy to cut slices to the size required to attach them to the slide for inspection. The process begins with tissue sampling.
Tissue can also be obtained from other animals such as rats or even snakes. Ideally, the blocks are only a few inches small, but this will depend on the network source and the type of extraction.
Immediately after excision, the tissue was immersed in a 10% neutral buffered formalin solution for about 18 to 24 hours. This will harden the fabric so it can last the next few steps.
In preparation for wax infiltration, the tissues are dried and cleaned, often using increased amounts of ethanol concentrate. This is then fed into IHC paraffin, which is specially sold to incorporate formaldehyde fabrics.
Fixation time is very important in FFPE – tissue repaired too early after excision may not be useful for molecular biology studies, but tissue must be repaired long enough to preserve it.